RSA defined

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Relative sidechain solvent accessibility : Three classes of relative sidechain solvent accessibility were defined based on the method of Lee and Richards (35). Residues with sidechain relative accessibilities of:

1. <17% were defined as inaccessible
2. 17–43% were defined as partially accessible
3. >43% were defined as accessible

These cut-offs were chosen based on an assessment of relative sidechain solvent accessibility values (36). The accessibility of each residue in a structure was calculated using the program psa (A. Sali, unpublished data).

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